A western blot, sometimes called a protein immunoblot, is an antibody-based technique used to detect the presence, size and abundance of specific proteins within a sample. The technique was developed ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

An interview with Ramya Prabakaran & Melody Chang from GenScript, at SfN 2018, discussing the major challenges for researchers carrying out western blotting, and how GenScript's automated systems are ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

Bio-Rad Laboratories, Inc. announces the launch of its Trans-Blot Turbo transfer system, a rapid western blotting instrument that allows researchers to efficiently transfer proteins from gel to blot ...

Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

In this editorial, Kenneth Oh overviews the two main normalization methods used to quantify western blots and discusses common errors to avoid. What is western blot normalization? Kenneth Oh is the ...

A classic laboratory technique, western blotting, has been used by researchers for decades, and it is nearly obsolete. "Biologists currently are stuck in a time warp—they basically use the same tools ...