Figure 1: Enzymatic cascade of the pyrosequencing reaction in the example of a bisulfite-treated template sequence, including a CpG position that is methylated on approximately 50% of all molecules.

Multiple fields of microbiology rely on techniques of microbial identification, such as in food microbiology for detecting contaminants, and in microbial ecology to characterize biodiversity. Image ...

Due to potential sequencing errors in pyrosequencing data, species richness and diversity indices of microbial systems can be miscalculated. The "traditional" sequence refinement method is not ...

In a little over seven hours, University of Illinois weed scientist Patrick Tranel got more genetic information about waterhemp than in two years time in a lab. The genetic information was obtained ...

Despite improvements in terms of sequence quality and price per basepair, Sanger sequencing remains restricted to screening of individual disease genes. The development of massively parallel ...

DNA methylation is a form of epigenetics in which gene expression is encouraged or inhibited by the addition of a methyl group on cytosines. It is highly implicated in development, cell ...